Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
The Human bFGF (FGF2) CLIA Kit is a cutting-edge tool designed for the precise measurement of basic fibroblast growth factor (bFGF or FGF2) levels in human samples. This kit offers superior sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.bFGF (FGF2) is a key growth factor that plays a critical role in cell proliferation, tissue regeneration, and wound healing. Dysregulation of bFGF levels has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.
As such, this CLIA kit serves as a valuable resource for investigating the role of bFGF in disease pathogenesis and developing potential therapeutic interventions.With its advanced technology and reliable performance, the Human bFGF (FGF2) CLIA Kit is an indispensable tool for researchers seeking to enhance their understanding of bFGF biology and its involvement in health and disease.
Product Name:
Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
SKU:
HUES00347
Target:
Human bFGF/FGF2 (Basic Fibroblast Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human bFGF/FGF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human bFGF/FGF2. You can calculate the concentration of Human bFGF/FGF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
85-100
90-106
Average (%)
99
91
97
1:4
Range (%)
97-112
94-109
93-106
Average (%)
105
101
98
1:8
Range (%)
94-108
99-113
93-107
Average (%)
100
105
99
1:16
Range (%)
87-102
96-113
96-111
Average (%)
95
104
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
98-115
105
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.16
84.03
280.73
39.06
77.9
290.55
Standard deviation
4.97
5.98
32.06
4.66
8.37
20.34
C V (%)
12.69
7.12
11.42
11.93
10.74
7.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human bFGF/FGF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human bFGF/FGF2 in samples. No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed.
