Human BLK Recombinant Protein (RPPB2443)
- SKU:
- RPPB2443
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P51451
- Research Area:
- Enzymes
Description
| Product Name: | Human BLK Recombinant Protein |
| Product Code: | RPPB2443 |
| Size: | 10µg |
| Species: | Human |
| Target: | BLK |
| Synonyms: | Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-Blk, BLK, MODY11, MGC10442. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | BLK protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAFSLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP |
B lymphoid kinase (BLK) is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains which contain consensus sequences of the Src protein tyrosine kinase family. BLK is a nonreceptor tyrosine-kinase belonging to the src family of proto-oncogenes which are typically involved in cell proliferation and differentiation. The BLK protein is expressed specifically in the B cell lineage and has a role in the signal-transduction pathway which is restricted to B lymphoid cells. In addition, BLK stimulates ins. synthesis and secretion in response to glucose and increases the expression of several pancreatic beta-cell transcription factors.
BLK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 525 amino acids (1-505 a.a.) and having a molecular mass of 59.8kDa.BLK is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes |
| UniProt Protein Details: | Protein type:Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Src family Chromosomal Location of Human Ortholog: 8p23-p22 Cellular Component: extrinsic to internal side of plasma membrane Molecular Function:protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding Biological Process: B cell receptor signaling pathway; innate immune response; positive regulation of insulin secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation Disease: Maturity-onset Diabetes Of The Young, Type 11 |
| NCBI Summary: | This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010] |
| UniProt Code: | P51451 |
| NCBI GenInfo Identifier: | 158936749 |
| NCBI Gene ID: | 640 |
| NCBI Accession: | P51451.3 |
| UniProt Secondary Accession: | P51451,Q16291, Q96IN1, |
| UniProt Related Accession: | P51451 |
| Molecular Weight: | 505 |
| NCBI Full Name: | Tyrosine-protein kinase Blk |
| NCBI Synonym Full Names: | BLK proto-oncogene, Src family tyrosine kinase |
| NCBI Official Symbol: | BLK�� |
| NCBI Official Synonym Symbols: | MODY11�� |
| NCBI Protein Information: | tyrosine-protein kinase Blk; p55-Blk; b lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase |
| UniProt Protein Name: | Tyrosine-protein kinase Blk |
| UniProt Synonym Protein Names: | B lymphocyte kinase; p55-Blk |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | BLK�� |
| UniProt Entry Name: | BLK_HUMAN |
