Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit
The Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit is specifically designed for the accurate measurement of BMP-7 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Bone Morphogenetic Protein 7, also known as osteogenic protein-1, is a key factor in bone and cartilage development, playing a crucial role in regulating cell differentiation and tissue regeneration. BMP-7 has also been implicated in various disease processes, including bone disorders, kidney diseases, and cancer, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.
With its advanced technology and dependable performance, the Human BMP-7 ELISA Kit from Assay Genie is an essential tool for researchers studying the role of BMP-7 in health and disease, providing valuable insights into its biological functions and potential clinical applications.
Product Name:
Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit
SKU:
HUES01300
Target:
Human BMP-7 (Bone Morphogenetic Protein 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BMP-7. You can calculate the concentration of Human BMP-7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
98-112
90-103
Average (%)
101
105
96
1:4
Range (%)
97-112
87-102
94-108
Average (%)
105
93
101
1:8
Range (%)
95-112
82-92
95-111
Average (%)
103
87
101
1:16
Range (%)
97-111
83-99
97-110
Average (%)
105
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.1
241.5
724.1
119.2
264.0
680.8
Standard deviation
6.9
9.7
27.5
8.0
13.7
31.3
C V (%)
6.27
4.02
3.8
6.71
5.19
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BMP-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-7 in samples. No significant cross-reactivity or interference between Human BMP-7 and analogues was observed.
