Human Brain Protein 44 Like Protein (BRP44L) ELISA Kit
The Human Brain Protein 44-Like Protein (BRP44L) ELISA Kit is a cutting-edge tool for the precise measurement of BRP44L levels in human biological samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research endeavors.BRP44L is a key protein associated with brain function, neuronal development, and neurodegenerative diseases. Its role in regulating synaptic transmission and plasticity makes it a valuable target for studying conditions like Alzheimer's disease, Parkinson's disease, and other neurological disorders.
The BRP44L ELISA Kit provides an opportunity to deepen our understanding of these diseases and explore potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human Brain Protein 44-Like Protein (BRP44L) ELISA Kit. Get your hands on this innovative assay and uncover new insights into brain health and disease mechanisms.
Product Name:
Human Brain Protein 44 Like Protein (BRP44L) ELISA Kit
Product Code:
HUDL00356
Size:
96 Assays
Target:
BRP44L
Synonyms:
CGI-129, MPC1, Mitochondrial Pyruvate Carrier Protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
