The Human C3a Complement Component 3a ELISA Kit is a valuable tool for researchers looking to accurately measure levels of the C3a complement component in human samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for serum, plasma, and cell culture supernatants. The C3a complement component is a key player in the immune system, playing a critical role in inflammation and immune response. Abnormal levels of C3a have been linked to various autoimmune diseases, infections, and inflammatory conditions, making it a crucial biomarker for understanding and studying these diseases. With easy-to-follow protocols and reliable performance, the Human C3a Complement Component 3a ELISA Kit is essential for researchers focused on immunology, inflammation, and disease mechanisms. This kit provides a convenient and accurate solution for measuring C3a levels in human samples, aiding in the advancement of research and potential therapeutic developments.
Product Name:
Human C3a (Complement Component 3a) ELISA Kit
SKU:
HUES01954
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
