Human Cardiotrophin Like Cytokine Factor 1 (CLCF1) ELISA Kit
The Human Cardiotrophin-like Cytokine Factor 1 (CLCF1) ELISA Kit is a powerful tool for detecting CLCF1 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers reliable and consistent results, making it ideal for various research applications.CLCF1 is a vital cytokine that plays a key role in inflammation and immune response regulation. It is implicated in a variety of diseases including autoimmune disorders, inflammatory conditions, and cancer.
Detecting CLCF1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Utilizing this ELISA kit enables researchers to accurately quantify CLCF1 levels in human samples, contributing to a better understanding of the cytokine's role in health and disease. With its ease of use and precision, the Human CLCF1 ELISA Kit is a valuable asset for research in immunology, inflammation, and disease pathology.
Product Name:
Human Cardiotrophin Like Cytokine Factor 1 (CLCF1) ELISA Kit
Product Code:
HUDL00583
Size:
96 Assays
Target:
CLCF1
Synonyms:
CLC, NR6, BSF3, CISS2, NNT1, Novel Neurotrophin-1, B Cell-Stimulating Factor-3
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
