The Human Casp14 (Caspase-14) CLIA Kit is a comprehensive and reliable tool for the quantitative measurement of Caspase-14 levels in human samples. This kit is specifically designed for the accurate detection of Caspase-14 in serum, plasma, and cell culture supernatants, providing researchers with valuable insights into the role of this protein in various physiological and pathological processes.Caspase-14 is a critical enzyme involved in the regulation of cell apoptosis and differentiation, making it a key player in cellular homeostasis. Aberrant Caspase-14 activity has been linked to a range of diseases, including skin disorders and inflammatory conditions, highlighting its potential as a therapeutic target.
With its high sensitivity and specificity, the Human Casp14 CLIA Kit offers precise and reproducible results, enabling researchers to conduct in-depth studies on Caspase-14 function and its implications in disease development. This kit is an indispensable tool for investigating the molecular mechanisms underlying various disorders and developing novel therapeutic strategies.
Product Name:
Human CASP14 (Caspase 14) CLIA Kit
SKU:
HUES00434
Target:
Human CASP14 (Caspase 14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP14. You can calculate the concentration of Human CASP14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
88-101
91-109
Average (%)
100
95
99
1:4
Range (%)
95-110
94-109
83-97
Average (%)
100
100
90
1:8
Range (%)
91-104
90-104
96-110
Average (%)
98
95
101
1:16
Range (%)
87-100
86-101
88-102
Average (%)
93
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.57
446.13
1741.95
220.81
407.28
1595.86
Standard deviation
26.76
38.99
153.47
22.24
44.19
112.51
C V (%)
12.13
8.74
8.81
10.07
10.85
7.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP14 in samples. No significant cross-reactivity or interference between Human CASP14 and analogues was observed.
