Human CCS Recombinant Protein (RPPB3001)
- SKU:
- RPPB3001
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O14618
Description
| Product Name: | Human CCS Recombinant Protein |
| Product Code: | RPPB3001 |
| Size: | 25µg |
| Species: | Human |
| Target: | CCS |
| Synonyms: | Superoxide dismutase copper chaperone, Copper chaperone for superoxide dismutase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CCS protein solution (1mg/1ml) contains 20 mM Tris-HCl buffer (pH8.0), 1mM DTT, 0.2M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASDSGNQGT LCTLEFAVQM TCQSCVDAVR KSLQGVAGVQ DVEVHLEDQM VLVHTTLPSQ EVQALLEGTG RQAVLKGMGS GQLQNLGAAV AILGGPGTVQ GVVRFLQLTP ERCLIEGTID GLEPGLHGLH VHQYGDLTNN CNSCGNHFNP DGASHGGPQD SDRHRGDLGN VRADADGRAI FRMEDEQLKV WDVIGRSLII DEGEDDLGRG GHPLSKITGN SGERLACGII ARSAGLFQNP KQICSCDGLT IWEERGRPIA GKGRKESAQP PAHL |
CCS is vital for the integration of copper into SOD-1, and as a result is needed for its enzymatic activity. CCS inhibits copper ions from binding to intracellular copper scavengers and provides the SOD-1 enzyme with the required copper cofactor. CCS escorts copper just to SOD-1 and is unable to deliver copper to proteins in the mitochondria, nucleus or secretory pathway. Although many tissues express CCS, the chaperone is most abundant in the kidney, liver and Purkinje cells in the neuropil of the central nervous system.
CCS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 294amino acids (1-274a.a.) and having a molecular wieght of 31.2kDa. The CCS is fused to a 20a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CCS: Delivers copper to copper zinc superoxide dismutase (SOD1). |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:copper ion transmembrane transporter activity; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity; protein disulfide oxidoreductase activity Biological Process: response to reactive oxygen species; removal of superoxide radicals; superoxide metabolic process; intracellular copper ion transport; positive regulation of oxidoreductase activity |
| NCBI Summary: | Copper chaperone for superoxide dismutase specifically delivers Cu to copper/zinc superoxide dismutase and may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14618 |
| NCBI GenInfo Identifier: | 20137612 |
| NCBI Gene ID: | 9973 |
| NCBI Accession: | O14618.1 |
| UniProt Secondary Accession: | O14618,Q2M366, Q8NEV0, |
| UniProt Related Accession: | O14618 |
| Molecular Weight: | 274 |
| NCBI Full Name: | Copper chaperone for superoxide dismutase |
| NCBI Synonym Full Names: | copper chaperone for superoxide dismutase |
| NCBI Official Symbol: | CCS�� |
| NCBI Protein Information: | copper chaperone for superoxide dismutase; superoxide dismutase copper chaperone |
| UniProt Protein Name: | Copper chaperone for superoxide dismutase |
| UniProt Synonym Protein Names: | Superoxide dismutase copper chaperone |
| Protein Family: | Capsanthin/capsorubin synthase |
| UniProt Gene Name: | CCS�� |
| UniProt Entry Name: | CCS_HUMAN |
