Human CD47 Recombinant Protein (RPPB3039)
- SKU:
- RPPB3039
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q08722
- Research Area:
- CD Antigens
Description
| Product Name: | Human CD47 Recombinant Protein |
| Product Code: | RPPB3039 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD47 |
| Synonyms: | CD47 Molecule, Antigenic Surface Determinant Protein OA3, CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer), Antigen Identified By Monoclonal Antibody 1D8, Integrin Associated Protein, Integrin-Associated Protein, Rh-Related Antigen, CD47 Glycoprotein, MER6, IAP, Integrin-Associated Signal Transducer, Leukocyte Surface Antigen CD47, CD47 Antigen, Protein MER6, OA3, CD47. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CD47 protein solution (1mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNEHHHHHH |
CD47, functions in cell adhesions by performing as an adhesion receptorfor THBS1 on platelets, furthermore CD47 plays a role in the modulation ofintegrins. In addition, CD47 takes a vital part in memory formation as well assynaptic plasticity in the hippocampus. Receptor for SIRPA avoids maturation ofimmature dendritic cells and inhibits cytokine production by mature dendriticcells. CD47 prevents premature elimination of red blood cells, and it is also implicatedin membrane permeability changes induced following virus infection.�
CD47 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (19-141 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 129 amino acids and having a molecular mass of 14.7kDa.CD47 shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q13.1-q13.2 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response |
| NCBI Summary: | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | Q08722 |
| NCBI GenInfo Identifier: | 1171879 |
| NCBI Gene ID: | 961 |
| NCBI Accession: | Q08722.1 |
| UniProt Related Accession: | Q08722 |
| Molecular Weight: | |
| NCBI Full Name: | Leukocyte surface antigen CD47 |
| NCBI Synonym Full Names: | CD47 molecule |
| NCBI Official Symbol: | CD47�� |
| NCBI Official Synonym Symbols: | IAP; OA3; MER6�� |
| NCBI Protein Information: | leukocyte surface antigen CD47 |
| UniProt Protein Name: | Leukocyte surface antigen CD47 |
| UniProt Synonym Protein Names: | Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47 |
| Protein Family: | IgE-binding protein |
| UniProt Gene Name: | CD47�� |
| UniProt Entry Name: | CD47_HUMAN |
