Human CD47 Recombinant Protein (RPPB5873)
- SKU:
- RPPB5873
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q08722
- Research Area:
- CD Antigens
Description
| Product Name: | Human CD47 Recombinant Protein |
| Product Code: | RPPB5873 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD47 |
| Synonyms: | CD47Molecule, Antigenic Surface Determinant Protein OA3, CD47 Antigen (Rh-RelatedAntigen, Integrin-Associated Signal Transducer), Antigen Identified byMonoclonal, Antibody 1D8, Integrin Associated Protein, Integrin-AssociatedProtein, Rh-Related, Antigen, CD47 Glycoprotein, MER6, IAP, Integrin-AssociatedSignal Transducer, Leukocyte Surface Antigen CD47, CD47 Antigen, Protein MER6, OA3. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CD47 proteinsolution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10%glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | QLLFNKTKSVEFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASLKMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNELEPKSCD KTHTCPPCPA PELLGGPSVFLFPPKPKDTL MISRTPEVTC VVVDVSHEDPEVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKCKVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNYKTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH |
CD47, functions in cell adhesions by performing as an adhesion receptor for THBS1 on platelets, furthermore CD47 plays a role in the modulation of integrins. In addition, CD47 takes a vital part in memory formation as well as synaptic plasticity in the hippocampus. Receptor for SIRPA avoids maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 prevents premature elimination of red blood cells, and it is also implicated in membrane permeability changes induced following virus infection.
CD47 HumanRecombinant produced in Sf9 Insect cell is a single, non-glycosylatedpolypeptide chain containing 362 amino acids (19-141aa.a) and having amolecular mass of 40.9kDa (Migrates at 40-57kDa on SDS-PAGE under reducingconditions).CD47 is fused to a 239 amino acid hIgG-His-tag atC-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q13.1-q13.2 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response |
| NCBI Summary: | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | Q08722 |
| NCBI GenInfo Identifier: | 1171879 |
| NCBI Gene ID: | 961 |
| NCBI Accession: | Q08722.1 |
| UniProt Related Accession: | Q08722 |
| Molecular Weight: | |
| NCBI Full Name: | Leukocyte surface antigen CD47 |
| NCBI Synonym Full Names: | CD47 molecule |
| NCBI Official Symbol: | CD47�� |
| NCBI Official Synonym Symbols: | IAP; OA3; MER6�� |
| NCBI Protein Information: | leukocyte surface antigen CD47 |
| UniProt Protein Name: | Leukocyte surface antigen CD47 |
| UniProt Synonym Protein Names: | Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47 |
| Protein Family: | IgE-binding protein |
| UniProt Gene Name: | CD47�� |
| UniProt Entry Name: | CD47_HUMAN |
