The Human Chemerin ELISA Kit (HUES01849) from AssayGenie is a cutting-edge research tool designed for the precise and accurate detection of chemerin levels in human samples. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Chemerin is a key protein involved in inflammation, adipogenesis, and metabolism, playing a critical role in conditions such as obesity, diabetes, and cardiovascular diseases.
By measuring chemerin levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially discover novel therapeutic targets.The AssayGenie Human Chemerin ELISA Kit includes all necessary components for easy and efficient sample analysis, making it a valuable addition to any research laboratory studying chemerin-related pathways and diseases. Order your kit today and unlock the potential of your research.
Product Name:
Human CHEM (Chemerin) ELISA Kit
SKU:
HUES01849
Target:
Human CHEM (Chemerin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CHEM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHEM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHEM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CHEM. You can calculate the concentration of Human CHEM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
90-101
87-101
Average (%)
103
96
94
1:4
Range (%)
91-102
79-93
82-93
Average (%)
97
86
88
1:8
Range (%)
86-102
84-96
85-98
Average (%)
93
90
92
1:16
Range (%)
88-102
84-97
82-95
Average (%)
94
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.51
4.45
0.45
1.53
4.6
Standard deviation
0.03
0.06
0.18
0.03
0.06
0.25
C V (%)
6.52
3.97
4.04
6.67
3.92
5.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CHEM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHEM in samples. No significant cross-reactivity or interference between Human CHEM and analogues was observed.
