Description
| Product Name: | Human CHMP6 Recombinant Protein |
| Product Code: | RPPB3178 |
| Size: | 20µg |
| Species: | Human |
| Target: | CHMP6 |
| Synonyms: | Charged multivesicular body protein 6, Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CHMP6 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMGNLFG RKKQSRVTEQ DKAILQLKQQ RDKLRQYQKR IAQQLERERA LARQLLRDGR KERAKLLLKK KRYQEQLLDR TENQISSLEA MVQSIEFTQI EMKVMEGLQF GNECLNKMHQ VMSIEEVERI LDETQEAVEY QRQIDELLAG SFTQEDEDAI LEELSAITQE QIELPEVPSE PLPEKIPENV PVKARPRQAE LVAAS |
Charged multivesicular body protein 6 (CHMP6) is a member of the SNF7 family. The CHMP6 protein is a core component of the endosomal sorting necessary for transport complex III (ESCRT-III) that is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) which are produced by invagination and scission from the limiting membrane of the endosome and generally are transported to lysosomes facilitating degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids.
CHMP6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-201 a.a) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear higher).CHMP6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CHMP6: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes. Belongs to the SNF7 family. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: membrane; late endosome membrane; cytosol Molecular Function:protein binding; protein N-terminus binding Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; vacuolar transport; viral infectious cycle; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis |
| NCBI Summary: | This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein family. Proteins in this family are part of the ESCRT-III (endosomal sorting complex required for transport III) which degrades surface receptors, and in biosynthesis of endosomes. [provided by RefSeq, Mar 2012] |
| UniProt Code: | Q96FZ7 |
| NCBI GenInfo Identifier: | 73917777 |
| NCBI Gene ID: | 79643 |
| NCBI Accession: | Q96FZ7.3 |
| UniProt Related Accession: | Q96FZ7 |
| Molecular Weight: | |
| NCBI Full Name: | Charged multivesicular body protein 6 |
| NCBI Synonym Full Names: | charged multivesicular body protein 6 |
| NCBI Official Symbol: | CHMP6�� |
| NCBI Official Synonym Symbols: | VPS20�� |
| NCBI Protein Information: | charged multivesicular body protein 6 |
| UniProt Protein Name: | Charged multivesicular body protein 6 |
| UniProt Synonym Protein Names: | Chromatin-modifying protein 6; Vacuolar protein sorting-associated protein 20; Vps20; hVps20 |
| Protein Family: | Charged multivesicular body protein |
| UniProt Gene Name: | CHMP6�� |
| UniProt Entry Name: | CHMP6_HUMAN |
