Product Name:
Description
Human CMV-IgG (cytomegalovirus-Immunoglobulin G) ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| | Human CMV-IgG (cytomegalovirus-Immunoglobulin G) ELISA Kit |
| Product Code: | HUFI03440 |
| Size: | 96 Assays |
| Alias: | CMV-IgG ELISA Kit |
| Detection Method: | Indirect ELISA - Biotin |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human CMV-IgG (cytomegalovirus-Immunoglobulin G) concentrations in serum plasma and other biological fluids. |
| Sensitivity: | < Qualitative |
| Range: | Qualitative |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery: | Matrices listed below were spiked with certain level of Human CMV-IgG (cytomegalovirus-Immunoglobulin G) and the recovery rates were calculated by comparing the measured value to the expected amount of Human CMV-IgG (cytomegalovirus-Immunoglobulin G) in samples. Enquire for more information. |
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CMV-IgG (cytomegalovirus-Immunoglobulin G) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Enquire for more information. |
| CV(%): | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protocol
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Before adding to the wells, equilibrate the TMB substrate for at least 30 mins at 37°C. When diluting samples and reagents, ensure they are mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 100µl of standard solutions into the standard wells. |
| 3. | Add 100µl of properly diluted Sample into the sample wells. |
| 4. | Seal the plate with a cover and incubate at 37 °C for 90 mins. |
| 5. | Wash: Repeat the aspiration/wash process for 2 times. |
| 6. | Add 100µl of Biotin-labelled antibody working solution into the bottom of each well (standard, test sample & zero wells) without touching the side walls. |
| 7. | Seal the plate with a cover and incubate at 37°C for 60 mins. |
| 8. | Wash: Remove the cover, and wash plate 3 times with Wash buffer. |
| 9. | HRP-Streptavidin Conjugate (SABC): Add 100µl of SABC Working Solution into each well, cover the plate and incubate at 37°C for 30 minutes |
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