Human COL1 alpha1 (Collagen Type I Alpha 1) CLIA Kit
The Human COL1A1 (Collagen Type I Alpha 1) CLIA Kit is specially designed for the precise measurement of collagen type I alpha 1 levels in human samples like serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Collagen type I alpha 1 is a key component of the extracellular matrix and is essential for maintaining the structural integrity and strength of tissues such as skin, bones, and tendons. Dysregulation of collagen type I alpha 1 is associated with various diseases, including fibrosis, osteoporosis, and connective tissue disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Whether you are investigating tissue repair mechanisms, studying fibrotic diseases, or exploring potential treatments for bone disorders, the Human COL1A1 CLIA Kit provides a reliable tool to accurately quantify collagen type I alpha 1 levels and advance your research efforts.
Product Name:
Human COL1 alpha1 (Collagen Type I Alpha 1) CLIA Kit
SKU:
HUES00524
Target:
Human COL1 alpha1 (Collagen Type I Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL1α1. You can calculate the concentration of Human COL1α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
84-97
97-111
Average (%)
95
90
105
1:4
Range (%)
93-106
86-101
90-102
Average (%)
98
93
96
1:8
Range (%)
90-102
100-115
101-115
Average (%)
97
106
107
1:16
Range (%)
93-106
91-103
85-98
Average (%)
100
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
103
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.69
471.48
1846.85
177.21
435.56
1730.77
Standard deviation
24.39
56.01
132.97
22.17
44.38
111.12
C V (%)
12.59
11.88
7.2
12.51
10.19
6.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL1α1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL1α1 in samples. No significant cross-reactivity or interference between Human COL1α1 and analogues was observed.
