Human COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit
The Human COL4 Alpha1 (Collagen Type IV Alpha 1) CLIA Kit is a reliable and accurate tool for measuring levels of collagen type IV alpha 1 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Collagen type IV alpha 1 is a key component of basement membranes and plays a critical role in maintaining tissue structure and function. Dysregulation of collagen type IV alpha 1 has been implicated in various diseases, including kidney disorders, cardiovascular diseases, and autoimmune conditions.
Therefore, this CLIA kit is essential for studying the role of collagen type IV alpha 1 in disease pathogenesis and for developing potential therapeutic interventions.Overall, the Human COL4 Alpha1 (Collagen Type IV Alpha 1) CLIA Kit provides researchers with a valuable tool for investigating the role of collagen type IV alpha 1 in health and disease, offering reliable and accurate measurements for a wide range of research applications.
Product Name:
Human COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit
SKU:
HUES00489
Target:
Human COL4 alpha1 (Collagen Type IV Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL4α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL4α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL4α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL4α1. You can calculate the concentration of Human COL4α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
88-99
103-120
Average (%)
103
94
109
1:4
Range (%)
92-110
101-119
98-114
Average (%)
100
109
107
1:8
Range (%)
94-107
87-102
93-107
Average (%)
100
93
100
1:16
Range (%)
89-103
104-121
91-109
Average (%)
94
110
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-99
90
EDTA plasma (n=5)
101-117
107
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
191.55
542.22
1620.45
199.4
585.88
1500.57
Standard deviation
23.54
42.56
130.77
20.58
47.69
137.9
C V (%)
12.29
7.85
8.07
10.32
8.14
9.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL4α1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL4α1 in samples. No significant cross-reactivity or interference between Human COL4α1 and analogues was observed.
