The Human CPB2 (Carboxypeptidase B2) ELISA Kit is a powerful tool for detecting CPB2 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and dependable results, making it suitable for a wide array of research applications. Carboxypeptidase B2 is an important enzyme involved in protein digestion and the regulation of peptide hormones. Dysregulation of CPB2 has been linked to various diseases, making it a valuable biomarker for studying conditions such as cancer, cardiovascular diseases, and inflammation.
By accurately measuring CPB2 levels, researchers can gain insights into disease processes and potentially identify new therapeutic strategies. Overall, the Human CPB2 ELISA Kit is an essential tool for researchers looking to explore the role of carboxypeptidase B2 in health and disease, ultimately advancing our understanding of these conditions and paving the way for innovative treatments.
Product Name:
Human CPB2/Carboxypeptidase B2 ELISA Kit
SKU:
HUFI00744
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
1.875 ng/mL
Range:
3.125-200 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
90-105%
88-99%
83-100%
EDTA Plasma (n = 5)
86-102%
85-95%
85-97%
Heparin Plasma (n = 5)
85-102%
89-97%
80-100%
Recovery:
Sample
1
2
Serum (n = 5)
86-101
91
EDTA Plasma (n = 5)
90-102
96
Heparin Plasma (n = 5)
85-102
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
