The Human CRP (C-Reactive Protein) CLIA Kit is specifically designed for the quantitative detection of CRP levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. CRP is a key biomarker of inflammation in the body and is often elevated in response to infection, injury, or other inflammatory conditions.
Monitoring CRP levels can provide valuable insight into the body's inflammatory response, making it a valuable tool for studying various diseases such as cardiovascular disease, autoimmune disorders, and infectious diseases. With its user-friendly protocol and quick turnaround time, the Human CRP CLIA Kit is an essential tool for researchers looking to study the role of CRP in health and disease.
Product Name:
Human CRP (C-Reactive Protein) CLIA Kit
SKU:
HUES00042
Target:
Human CRP (C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
84-99
87-100
Average (%)
102
91
92
1:4
Range (%)
92-105
89-100
86-97
Average (%)
98
95
91
1:8
Range (%)
100-112
87-97
91-105
Average (%)
105
92
99
1:16
Range (%)
91-102
91-104
85-99
Average (%)
96
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
3.08
7.6
0.82
3.31
7.08
Standard deviation
0.11
0.35
0.66
0.09
0.27
0.53
C V (%)
12.09
11.36
8.68
10.98
8.16
7.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
