The Human CTSK (Cathepsin K) ELISA Kit is specifically designed for the accurate quantification of Cathepsin K levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Cathepsin K is a key enzyme involved in bone remodeling and is also implicated in various pathological conditions such as osteoporosis and certain types of cancer.
By measuring Cathepsin K levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.With this ELISA kit, researchers can confidently study the role of Cathepsin K in various biological processes and disease settings, advancing our understanding of its function and potential implications for clinical applications.
Product Name:
Human CTSK (Cathepsin K) ELISA Kit
SKU:
HUES01826
Target:
Human CTSK (Cathepsin K)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTSK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTSK. You can calculate the concentration of Human CTSK in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
91-104
97-113
Average (%)
105
98
103
1:4
Range (%)
91-107
83-93
86-100
Average (%)
98
88
91
1:8
Range (%)
88-102
82-94
86-100
Average (%)
94
87
93
1:16
Range (%)
88-101
81-92
87-100
Average (%)
95
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.57
4.41
0.48
1.52
4.13
Standard deviation
0.03
0.08
0.17
0.03
0.07
0.16
C V (%)
6.52
5.1
3.85
6.25
4.61
3.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTSK concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed.
