The Human CTSL (Cathepsin L) CLIA Kit is a highly reliable and accurate tool designed for the detection of Cathepsin L levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for a variety of research applications.Cathepsin L is an important enzyme involved in various physiological processes, including protein degradation, antigen processing, and tissue remodeling. Dysregulation of Cathepsin L has been linked to several diseases, such as cancer, arthritis, and cardiovascular disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic development.
This innovative CLIA kit provides researchers with a valuable tool for studying the role of Cathepsin L in disease pathogenesis and identifying potential therapeutic targets. With its user-friendly protocol and reliable performance, the Human CTSL CLIA Kit is a must-have for researchers in the field of molecular biology and biomedicine.
Product Name:
Human CTSL (Cathepsin L) CLIA Kit
SKU:
HUES00442
Target:
Human CTSL (Cathepsin L)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTSL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTSL. You can calculate the concentration of Human CTSL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
95-107
100-118
Average (%)
106
101
107
1:4
Range (%)
86-98
87-102
90-102
Average (%)
91
93
96
1:8
Range (%)
101-117
84-99
83-99
Average (%)
107
90
90
1:16
Range (%)
92-106
85-97
98-116
Average (%)
98
90
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.09
131.66
462.08
50.59
142.21
465.5
Standard deviation
4.77
15.1
34.38
5.89
12.84
33.98
C V (%)
10.35
11.47
7.44
11.64
9.03
7.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTSL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSL in samples. No significant cross-reactivity or interference between Human CTSL and analogues was observed.
