The CTSL ELISA Kit is designed for the quantitative detection of Cathepsin L (CTSL) levels in various human biological samples. CTSL is a critical protease enzyme implicated in a multitude of cellular activities, including protein degradation, cell growth, and apoptosis. It has a significant role in immunity, and its dysregulation can be associated with various pathological conditions such as cancer, cardiovascular disorders, and autoimmune diseases.
Hence, precise detection of CTSL is crucial for understanding its role in these pathological mechanisms and for the development of targeted therapeutic strategies. Assay Genie's CTSL ELISA Kit offers exceptional sensitivity and specificity, guaranteeing reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures sturdy performance and is simple to use, making it an excellent choice for both research and clinical applications. Rely on Assay Genie's CTSL ELISA Kit for precise and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human CTSL (Cathepsin L) ELISA Kit
SKU:
AEES00028
Target:
Human CTSL (Cathepsin L)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTSL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTSL. You can calculate the concentration of Human CTSL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
93-109
96-111
Average (%)
100
101
102
1:4
Range (%)
91-105
80-92
87-100
Average (%)
98
87
93
1:8
Range (%)
87-102
88-101
82-96
Average (%)
93
93
89
1:16
Range (%)
89-104
79-93
82-95
Average (%)
97
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.7
575.05
1881.18
171.62
561.21
1697.86
Standard deviation
11.11
28.69
87.47
11.5
30.25
66.56
C V (%)
5.92
4.99
4.65
6.7
5.39
3.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTSL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSL in samples. No significant cross-reactivity or interference between Human CTSL and analogues was observed.
