Human Cyclin Dependent Kinase 16 (CDK16) ELISA Kit
The Human Cyclin-Dependent Kinase 16 (CDK16) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CDK16 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a variety of research applications.CDK16 is a key protein involved in cell cycle regulation and has been implicated in various diseases such as cancer and neurological disorders.
Understanding the role of CDK16 in these conditions can offer valuable insights for developing targeted therapies and advancing clinical research.With its advanced technology and precise measurement capabilities, the Human CDK16 ELISA Kit from AssayGenie is a trusted solution for researchers seeking to explore the functions and implications of CDK16 in human health and disease.
Product Name:
Human Cyclin Dependent Kinase 16 (CDK16) ELISA Kit
Product Code:
HUDL00512
Size:
96 Assays
Target:
CDK16
Synonyms:
PCTK1, PCTAIRE, PCTAIRE1, PCTGAIRE, Serine/Threonine-Protein Kinase PCTAIRE-1, PCTAIRE-motif protein kinase 1, Cell division protein kinase 16
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
