Human Cyclophilin E Recombinant Protein (RPPB1541)
- SKU:
- RPPB1541
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UNP9
- Research Area:
- Enzymes
Description
| Product Name: | Human Cyclophilin E Recombinant Protein |
| Product Code: | RPPB1541 |
| Size: | 25µg |
| Species: | Human |
| Target: | Cyclophilin E |
| Synonyms: | Peptidyl-prolyl cis-trans isomerase E, PPIase E, Rotamase E, Cyclophilin-33, PPIE, peptidylprolyl isomerase E, CYP33, Cyclophilin E, CYP-33, MGC3736, MGC111222. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | Cyclophilin-E solution containing 20mM Tris pH-8. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV |
| Biological Activity: | Specific activity is > 210 nmoles/min/ug, and is defined as the amount of enzyme that cleave 1umole of suc-AAFP-pNA per minute at 1C in Tris-HCl pH8.0 using chymotrypsin. |
Cyclophilin-E is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and speeds up the protein folding. Cyclophilin-E contains a highly conserved cyclophilin domain in addition to a RNA-binding domain. Cyclophilin-E exhibits PPIase activity, protein folding activities and possess RNA-binding activity. Cyclophilin-E contains 2 RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region.
Cyclophilin-E Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-301 a.a.) and having a molecular mass of 37.5 kDa. Cyclophilin-E is fused to a 36 amino acids long His Tag at N-terminus and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPIE: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIase E subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Spliceosome; Cyclophilin; RNA splicing; EC 5.2.1.8; Isomerase Chromosomal Location of Human Ortholog: 1p32 Cellular Component: nucleoplasm; nucleus Molecular Function:cyclosporin A binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; RNA binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; transcription-coupled nucleotide-excision repair |
| NCBI Summary: | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010] |
| UniProt Code: | Q9UNP9 |
| NCBI GenInfo Identifier: | 13124097 |
| NCBI Gene ID: | 10450 |
| NCBI Accession: | Q9UNP9.1 |
| UniProt Secondary Accession: | Q9UNP9,O43634, O43635, Q32Q72, Q3S611, Q5TGA0, Q5TGA2 Q5TGA3, Q9UIZ5, B2R971, |
| UniProt Related Accession: | Q9UNP9 |
| Molecular Weight: | 34,991 Da |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase E |
| NCBI Synonym Full Names: | peptidylprolyl isomerase E |
| NCBI Official Symbol: | PPIE�� |
| NCBI Official Synonym Symbols: | CYP33; CYP-33�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase E |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase E |
| UniProt Synonym Protein Names: | Cyclophilin E; Cyclophilin-33; Rotamase E |
| UniProt Gene Name: | PPIE�� |
| UniProt Entry Name: | PPIE_HUMAN |
