Human D betaH (Dopamine- beta-Hydroxylase) CLIA Kit
The Human D-BetaH Dopamine Beta Hydroxylase CLIA Kit is a reliable and highly sensitive assay designed for the accurate detection of D-BetaH levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring dependable results for a variety of research applications.D-BetaH is an important enzyme involved in the synthesis of dopamine, playing a crucial role in the nervous system. Dysregulation of D-BetaH activity has been linked to various neurological disorders, such as Parkinson's disease and schizophrenia.
Therefore, accurate measurement of D-BetaH levels is essential for understanding the pathophysiology of these conditions and developing potential therapeutic interventions.Overall, the Human D-BetaH Dopamine Beta Hydroxylase CLIA Kit is a valuable tool for researchers studying neurobiology and related disorders, providing reliable data for further exploration and discovery.
Product Name:
Human D betaH (Dopamine- beta-Hydroxylase) CLIA Kit
SKU:
HUES00699
Target:
Human D betaH (Dopamine- beta-Hydroxylase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DβH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DβH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DβH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DβH. You can calculate the concentration of Human DβH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-111
102-117
Average (%)
92
101
109
1:4
Range (%)
98-117
99-113
94-106
Average (%)
106
107
100
1:8
Range (%)
88-101
87-100
88-100
Average (%)
93
93
94
1:16
Range (%)
102-116
85-99
90-102
Average (%)
108
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.22
6.94
0.86
2.43
7.09
Standard deviation
0.12
0.18
0.8
0.11
0.23
0.48
C V (%)
13.04
8.11
11.53
12.79
9.47
6.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DβH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DβH in samples. No significant cross-reactivity or interference between Human DβH and analogues was observed.
