The Human D-Lactate Dehydrogenase (D-LDH) ELISA Kit from Assay Genie is an innovative tool for the accurate measurement of D-LDH levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.D-Lactate Dehydrogenase is an important enzyme involved in metabolic processes, particularly in the conversion of lactate to pyruvate. Dysregulation of D-LDH levels has been associated with various pathological conditions such as liver disease, muscle injury, and metabolic disorders.
This ELISA kit allows researchers to study the role of D-LDH in these conditions and explore potential therapeutic interventions.With its user-friendly protocol and quick assay time, the Human D-Lactate Dehydrogenase ELISA Kit from Assay Genie is a valuable tool for researchers interested in studying the functions and implications of D-LDH in human health and disease.
Product Name:
Human D-LDH (D-Lactate Dehydrogenase) ELISA Kit
SKU:
HUES01994
Target:
Human D-LDH (D-Lactate Dehydrogenase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D-LDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human D-LDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human D-LDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human D-LDH. You can calculate the concentration of Human D-LDH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
88-100
88-104
Average (%)
102
94
95
1:4
Range (%)
100-113
86-98
96-113
Average (%)
107
92
103
1:8
Range (%)
97-111
85-97
90-104
Average (%)
102
90
97
1:16
Range (%)
97-111
81-92
95-110
Average (%)
104
86
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-97
90
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5
15.8
36.6
4.5
14.3
39.9
Standard deviation
0.3
0.9
1.7
0.2
0.7
1.4
C V (%)
6
5.7
4.64
4.44
4.9
3.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human D-LDH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human D-LDH in samples. No significant cross-reactivity or interference between Human D-LDH and analogues was observed.
