The Human DKK1 (Dickkopf-related protein 1) ELISA Kit is designed for the precise measurement of DKK1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results, making it a valuable tool for various research purposes.DKK1 is a key regulatory protein involved in the Wnt signaling pathway, influencing processes such as cell differentiation, proliferation, and tissue development.
Dysregulation of DKK1 has been implicated in various diseases, including cancer, osteoporosis, and Alzheimer's disease, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.With its advanced technology and reliable performance, the Human DKK1 ELISA Kit from Assay Genie provides researchers with a versatile and efficient solution for studying the role of DKK1 in health and disease.
Product Name:
Human DKK1 (Dickkopf Related Protein 1) ELISA Kit
SKU:
HUES01344
Target:
Human DKK1 (Dickkopf Related Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DKK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DKK1. You can calculate the concentration of Human DKK1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
90-106
90-104
Average (%)
103
98
97
1:4
Range (%)
86-99
81-94
85-98
Average (%)
93
88
90
1:8
Range (%)
89-101
84-98
82-97
Average (%)
96
91
89
1:16
Range (%)
92-104
86-99
87-99
Average (%)
98
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.7
259.7
766.5
107.28
279.39
742.61
Standard deviation
6.53
12.21
33.88
5.73
12.13
39.66
C V (%)
6.12
4.7
4.42
5.34
4.34
5.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DKK1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DKK1 in samples. No significant cross-reactivity or interference between Human DKK1 and analogues was observed.
