Human Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) ELISA Kit
The Human Dolichyl phosphate N-acetylglucosaminephosphotransferase 1 (DPAGT1) ELISA Kit is specifically designed to accurately measure levels of DPAGT1 in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and consistent results for various research purposes. DPAGT1 is a key enzyme involved in the biosynthesis of dolichol-phosphate-glucose, an essential step in protein glycosylation.
Dysfunction of DPAGT1 has been linked to various diseases such as congenital disorders of glycosylation, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies. Overall, the Human DPAGT1 ELISA Kit provides researchers with a reliable tool to accurately measure DPAGT1 levels, offering valuable insights into the role of this enzyme in health and disease.
Product Name:
Human Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
