The Human E-Cad (E-cadherin) CLIA Kit is specifically designed for the precise measurement of E-cadherin levels in human samples, including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reproducible results, making it an invaluable tool for a variety of research applications.E-cadherin is a key protein involved in cell adhesion and maintenance of tissue structure. Dysregulation of E-cadherin expression has been linked to various diseases, including cancer and inflammatory conditions.
Monitoring E-cadherin levels can provide valuable insights into disease progression and potential treatment options.Overall, the Human E-Cad (E-cadherin) CLIA Kit is essential for researchers studying cell adhesion, tissue organization, and disease mechanisms related to E-cadherin expression. Its reliable performance and ease of use make it a must-have tool for any laboratory conducting research in this field.
Product Name:
Human E-Cad (E-Cadherin) CLIA Kit
SKU:
HUES00014
Target:
Human E-Cad (E-Cadherin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human E-Cad. You can calculate the concentration of Human E-Cad in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
95-110
89-101
Average (%)
95
101
94
1:4
Range (%)
88-102
85-97
88-100
Average (%)
95
92
94
1:8
Range (%)
90-106
99-115
103-118
Average (%)
97
105
109
1:16
Range (%)
86-99
101-114
96-110
Average (%)
92
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.29
314.83
843.78
96.89
320.38
912.98
Standard deviation
8.35
34.41
76.36
9.62
32.36
77.33
C V (%)
8.58
10.93
9.05
9.93
10.1
8.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human E-Cad concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human E-Cad in samples. No significant cross-reactivity or interference between Human E-Cad and analogues was observed.
