Human ECI1 Recombinant Protein (RPPB1608)
- SKU:
- RPPB1608
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P42126
- Research Area:
- Enzymes
Description
| Product Name: | Human ECI1 Recombinant Protein |
| Product Code: | RPPB1608 |
| Size: | 10µg |
| Species: | Human |
| Target: | ECI1 |
| Synonyms: | Enoyl-CoA delta isomerase 1, mitochondrial, 3,2-trans-enoyl-CoA isomerase, Enoyl-CoA Delta Isomerase 1, Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ECI1 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK SLQMYLERLK EEKG |
Enoyl-CoA Delta Isomerase 1 (ECI1) is a main mitochondrial enzyme which takes part in beta-oxidation of unsaturated fatty acids. ECI1 is a member of the hydratase/isomerase superfamily. ECI1 catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters to the 2-trans-enoylCoA intermediates.
ECI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (42-302 a.a.) and having a molecular mass of 31.1kDa. ECI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 5.3.3.8; Isomerase; Mitochondrial; Lipid Metabolism - fatty acid Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane Molecular Function:dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process |
| NCBI Summary: | This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010] |
| UniProt Code: | P42126 |
| NCBI GenInfo Identifier: | 1169204 |
| NCBI Gene ID: | 1632 |
| NCBI Accession: | P42126.1 |
| UniProt Related Accession: | P42126 |
| Molecular Weight: | |
| NCBI Full Name: | Enoyl-CoA delta isomerase 1, mitochondrial |
| NCBI Synonym Full Names: | enoyl-CoA delta isomerase 1 |
| NCBI Official Symbol: | ECI1�� |
| NCBI Official Synonym Symbols: | DCI�� |
| NCBI Protein Information: | enoyl-CoA delta isomerase 1, mitochondrial |
| UniProt Protein Name: | Enoyl-CoA delta isomerase 1, mitochondrial |
| UniProt Synonym Protein Names: | 3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase |
| UniProt Gene Name: | ECI1�� |
| UniProt Entry Name: | ECI1_HUMAN |
