The Human ENG (Endoglin) ELISA Kit offered by Assay Genie is meticulously designed for the precise quantitative detection of Endoglin (ENG) levels in diverse biological samples.
Endoglin, a crucial component of vascular and tissue homeostasis, is involved in modulating cellular proliferation, differentiation, and migration. Notably, Endoglin plays a pivotal role in angiogenesis and endothelial function, making it a compelling target for cardiovascular and vascular studies. Our ENG ELISA Kit promises outstanding sensitivity and specificity, ensuring accurate and reproducible measurement of Endoglin concentrations. Crafted under stringent quality control standards, this kit delivers robust and dependable performance, offering researchers a valuable tool to advance their investigations. Trust in Assay Genie's ENG ELISA Kit for reliable and insightful results in your research endeavors.
Product Name:
Human ENG (Endoglin) ELISA Kit
SKU:
AEES00173
Target:
Human ENG (Endoglin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ENG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ENG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ENG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ENG. You can calculate the concentration of Human ENG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
95-111
84-97
Average (%)
101
101
91
1:4
Range (%)
94-109
96-107
86-97
Average (%)
101
102
91
1:8
Range (%)
90-103
93-107
87-101
Average (%)
96
99
93
1:16
Range (%)
94-107
91-105
80-92
Average (%)
99
96
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
92-109
100
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.19
4.76
0.46
1.22
4.32
Standard deviation
0.03
0.05
0.17
0.03
0.07
0.16
C V (%)
5.59
4.32
3.56
6.07
5.34
3.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ENG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ENG in samples. No significant cross-reactivity or interference between Human ENG and analogues was observed.
