Human EPCR (Endothelial Protein C Receptor) CLIA Kit
The Human EPCR (Endothelial Protein C Receptor) CLIA Kit is an essential tool for researchers looking to accurately measure EPCR levels in human samples. EPCR is a critical component in the coagulation and inflammation pathways, making it a key player in various diseases such as sepsis and thrombosis.This kit offers high sensitivity and specificity, ensuring precise and reliable results in serum, plasma, and cell culture supernatants.
With its easy-to-use format and quick assay time, researchers can confidently study EPCR levels and its implications in various conditions.By accurately measuring EPCR levels, researchers can gain valuable insights into the role of this receptor in disease progression and potentially develop new therapeutic interventions. Trust the Human EPCR CLIA Kit for accurate and reproducible results in your research endeavors.
Product Name:
Human EPCR (Endothelial Protein C Receptor) CLIA Kit
SKU:
HUES00063
Target:
Human EPCR (Endothelial Protein C Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPCR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPCR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPCR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPCR. You can calculate the concentration of Human EPCR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
93-108
102-115
Average (%)
103
99
109
1:4
Range (%)
96-113
101-119
86-99
Average (%)
104
109
91
1:8
Range (%)
87-98
87-99
102-119
Average (%)
92
92
110
1:16
Range (%)
93-106
91-108
101-119
Average (%)
99
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
91
EDTA plasma (n=5)
97-113
105
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.09
4.46
0.57
0.99
4.37
Standard deviation
0.07
0.08
0.28
0.06
0.1
0.37
C V (%)
12.96
7.34
6.28
10.53
10.1
8.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPCR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPCR in samples. No significant cross-reactivity or interference between Human EPCR and analogues was observed.
