The Human F13 (Coagulation Factor 13) CLIA Kit is specifically designed for the quantitative detection of F13 levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring the accurate and reliable measurement of F13 levels for research purposes.Coagulation Factor 13, also known as F13, is a vital component in the blood clotting process, playing a significant role in maintaining hemostasis and preventing excessive bleeding. It is essential for wound healing and tissue repair, making it a valuable biomarker for studying various coagulation disorders and monitoring patient response to treatment.
This CLIA kit provides researchers with a powerful tool for investigating the role of F13 in clot formation and fibrinolysis, as well as its potential implications in conditions such as thrombosis, hemophilia, and other coagulation-related disorders. With its accuracy and precision, the Human F13 CLIA Kit is a valuable asset for any laboratory conducting research in hemostasis and thrombosis.
Product Name:
Human F13 (Coagulation Factor 13) CLIA Kit
SKU:
HUES00478
Target:
Human F13 (Coagulation Factor 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F13. You can calculate the concentration of Human F13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
94-108
89-105
Average (%)
102
101
96
1:4
Range (%)
89-107
85-97
92-103
Average (%)
97
91
98
1:8
Range (%)
99-111
97-113
97-109
Average (%)
104
103
103
1:16
Range (%)
92-108
94-109
100-120
Average (%)
99
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
98-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.4
108.68
292.82
35.82
106.16
299.07
Standard deviation
3.74
9.98
23.51
4.62
7.43
28.35
C V (%)
10.0
9.18
8.03
12.9
7.0
9.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F13 in samples. No significant cross-reactivity or interference between Human F13 and analogues was observed.
