Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
The Human FABP3 (Fatty Acid Binding Protein 3) Muscle and Heart CLIA Kit is a reliable tool for the quantitative detection of FABP3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FABP3 is a key protein involved in the transport and metabolism of fatty acids in muscle and heart tissues.
Dysregulation of FABP3 has been linked to various cardiovascular disorders, making it an important biomarker for studying these conditions and exploring potential therapeutic interventions.By utilizing the Human FABP3 Muscle and Heart CLIA Kit, researchers can gain valuable insights into the role of FABP3 in health and disease, paving the way for advancements in personalized medicine and targeted treatment approaches.
Product Name:
Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
SKU:
HUES00811
Target:
Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FABP3. You can calculate the concentration of Human FABP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
88-101
87-100
Average (%)
96
96
94
1:4
Range (%)
93-103
89-104
88-104
Average (%)
98
96
95
1:8
Range (%)
94-110
97-111
89-103
Average (%)
101
104
94
1:16
Range (%)
101-117
89-104
92-107
Average (%)
107
96
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
98-115
105
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.64
225.2
728.63
93.93
228.99
730.08
Standard deviation
9.67
15.76
83.57
11.67
23.7
78.63
C V (%)
9.42
7.0
11.47
12.42
10.35
10.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FABP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP3 in samples. No significant cross-reactivity or interference between Human FABP3 and analogues was observed.
