Human FABP5 (Fatty Acid Binding Protein 5, Epidermal) ELISA Kit
The Human FABP5 (Fatty Acid Binding Protein 5) ELISA Kit is specifically designed for the accurate measurement of FABP5 levels in human epidermal samples. This kit offers high sensitivity and specificity, allowing for precise and consistent results for various research applications.FABP5 is a key protein involved in fatty acid transport and metabolism in the skin, playing a crucial role in maintaining skin barrier function and regulating lipid homeostasis.
Dysregulation of FABP5 has been linked to skin disorders such as psoriasis, atopic dermatitis, and skin cancer, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.With its reliable performance and user-friendly protocol, the Human FABP5 ELISA Kit is an essential tool for researchers investigating the role of FABP5 in skin physiology and pathology.
Product Name:
Human FABP5 (Fatty Acid Binding Protein 5, Epidermal) ELISA Kit
SKU:
HUES02181
Target:
Human FABP5 (Fatty Acid Binding Protein 5, Epidermal )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FABP5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FABP5. You can calculate the concentration of Human FABP5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
84-96
85-96
Average (%)
97
91
90
1:4
Range (%)
88-99
87-98
85-99
Average (%)
93
92
92
1:8
Range (%)
91-105
84-95
83-95
Average (%)
96
90
88
1:16
Range (%)
91-107
79-92
87-99
Average (%)
98
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-111
101
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
2.47
8.12
1.02
2.43
7.93
Standard deviation
0.06
0.12
0.42
0.05
0.13
0.38
C V (%)
5.83
4.86
5.17
4.9
5.35
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FABP5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed.
