Human FABP6 (Fatty Acid Binding Protein 6, Ileal) CLIA Kit
The Human FABP6 (Fatty Acid Binding Protein 6) CLIA Kit is a reliable tool for the quantitative measurement of FABP6 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.FABP6, also known as ileal lipid-binding protein, plays a crucial role in the absorption and transport of bile acids in the intestine.
Dysregulation of FABP6 has been associated with various gastrointestinal disorders and metabolic diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutics.Overall, the Human FABP6 CLIA Kit offers researchers a powerful tool for investigating the role of FABP6 in health and disease, providing valuable insights into intestinal lipid metabolism and potential therapeutic targets.
Product Name:
Human FABP6 (Fatty Acid Binding Protein 6, Ileal) CLIA Kit
SKU:
HUES00806
Target:
Human FABP6 (Fatty Acid Binding Protein 6, Ileal)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FABP6. You can calculate the concentration of Human FABP6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
100-115
102-120
Average (%)
107
105
110
1:4
Range (%)
93-110
94-108
93-109
Average (%)
100
99
100
1:8
Range (%)
95-108
94-107
99-115
Average (%)
101
101
107
1:16
Range (%)
87-99
95-111
98-116
Average (%)
94
102
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
98
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.45
225.8
693.13
101.19
234.01
705.45
Standard deviation
9.63
17.97
54.27
11.32
22.68
61.02
C V (%)
9.98
7.96
7.83
11.19
9.69
8.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FABP6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP6 in samples. No significant cross-reactivity or interference between Human FABP6 and analogues was observed.
