FcER1 / Fc epsilon RI binds to the Fc region of immunoglobulins epsilon, and is responsible for initiating the allergic response. FcER1 / Fc epsilon RI receptor also induces the secretion of important lymphokines. Diseases associated with FcER1 / Fc epsilon RI include chronic spontaneous urticaria. The Assay Genie Human FcER1 / Fc epsilon RI ELISA Kit is a highly sensitive assay for the quantitative measurement of FcER1 / Fc epsilon RI in serum, plasma, cell culture supernatant and tissue samples.
Description
Human FcER1 / Fc epsilon RI ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human FcER1 / Fc epsilon I ELISA Kit |
| Product Code: | HUFI02067 |
| Size: | 96 Assays |
| Alias: | FCER1A, FCE1A, FcER1, Fc-Epsilon RI-alpha, IgE Fc receptor subunit alpha |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in virto quantitative determination of Human FCER1A concetrations in serum plasma and the other biological fluids. |
| Sensitivity: | 18.75pg/ml |
| Range: | 31.25-2000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
Recovery:
Matrices listed below were spiked with certain level of Human FCER1A and the recovery rates were calculated by comparing the measured value to the expected amount of Human FCER1 in samples.
| Matrix | Recovery Range (%) | Average (%) |
| serum (n=5) | 85-98 | 93 |
| EDTA plasma (n=5) | 86-104 | 99 |
| UFH plasma (n=5) | 89-105 | 96 |
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human FCER1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
| serum (n=5) | 91-105% | 87-105% | 89-104% |
| EDTA plasma (n=5) | 88-100% | 89-99% | 85-99% |
| UFH plasma (n=5) | 87-97% | 81-97% | 81-98% |
CV(%):
Intra - Assay: CV<8%
Intra - Assay: CV<10%
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
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