Human FGF21 (Fibroblast Growth Factor 21) CLIA Kit
The Human FGF21 (Fibroblast Growth Factor 21) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of FGF21 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.FGF21 is a key regulator of metabolism and energy homeostasis, playing a critical role in conditions such as obesity, diabetes, and metabolic syndrome.
By measuring FGF21 levels, researchers can gain valuable insights into these metabolic disorders and potential therapeutic targets.Overall, the Human FGF21 CLIA Kit is a valuable tool for studying the role of FGF21 in metabolic diseases and developing novel treatment strategies.
Product Name:
Human FGF21 (Fibroblast Growth Factor 21) CLIA Kit
SKU:
HUES00072
Target:
Human FGF21 (Fibroblast Growth Factor 21)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGF21. You can calculate the concentration of Human FGF21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
97-112
88-105
Average (%)
100
103
96
1:4
Range (%)
101-116
96-112
95-112
Average (%)
108
104
102
1:8
Range (%)
98-113
103-115
83-95
Average (%)
105
108
90
1:16
Range (%)
90-104
95-107
87-102
Average (%)
98
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
97-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.32
81.85
297.58
39.7
80.4
316.22
Standard deviation
4.68
7.07
23.48
3.5
7.26
28.62
C V (%)
11.9
8.64
7.89
8.82
9.03
9.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGF21 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed.
