The Human FGL1 (Fibrinogen-Like Protein 1) CLIA Kit is a specialized assay designed for the precise measurement of FGL1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FGL1 is a member of the fibrinogen-related protein superfamily and is known to play a key role in regulating immune responses, liver regeneration, and tumor progression.
Elevated levels of FGL1 have been associated with certain diseases such as liver cancer, making it a valuable biomarker for research studies and potential therapeutic interventions.The Human FGL1 CLIA Kit from Assay Genie provides researchers with a reliable and advanced tool for studying the functions and mechanisms of FGL1 in various physiological and pathological processes. Get your hands on this essential kit today and unlock new insights into the role of FGL1 in human health and disease.
Product Name:
Human FGL1 (Fibrinogen Like Protein 1) CLIA Kit
SKU:
HUES00938
Target:
Human FGL1 (Fibrinogen Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGL1. You can calculate the concentration of Human FGL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
92-108
87-99
Average (%)
96
100
94
1:4
Range (%)
93-107
93-110
85-97
Average (%)
101
101
92
1:8
Range (%)
102-115
98-116
96-108
Average (%)
108
106
102
1:16
Range (%)
89-102
92-106
96-110
Average (%)
94
99
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
102
EDTA plasma (n=5)
98-115
105
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
111.02
254.74
773.58
119.04
229.97
779.92
Standard deviation
9.81
27.05
49.04
15.34
17.89
62.86
C V (%)
8.84
10.62
6.34
12.89
7.78
8.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGL1 in samples. No significant cross-reactivity or interference between Human FGL1 and analogues was observed.
Sandra Schwarz et al.
Peptide-stimulation of autologous T cells reverts immune checkpoint inhibitor resistance of hypermutated colorectal cancer cell
