The Human FN (Fibronectin) CLIA Kit is a cutting-edge assay designed for the precise measurement of fibronectin levels in human samples, including serum, plasma, and cell culture supernatants. With its advanced technology, this kit delivers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Fibronectin is a pivotal glycoprotein that plays a crucial role in cell adhesion, migration, and tissue remodeling. Its presence is implicated in numerous diseases, including cancer, fibrosis, and wound healing, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
By utilizing the Human FN (Fibronectin) CLIA Kit, researchers can gain valuable insights into the role of fibronectin in disease pathology and potentially identify new targets for treatment. With its reliable performance and ease of use, this kit is a valuable tool for advancing research in the field of fibronectin biology.
Product Name:
Human FN (Fibronectin) CLIA Kit
SKU:
HUES00153
Target:
Human FN (Fibronectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FN. You can calculate the concentration of Human FN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
93-107
99-113
Average (%)
103
98
106
1:4
Range (%)
90-101
91-104
89-106
Average (%)
95
97
97
1:8
Range (%)
101-118
100-113
100-116
Average (%)
108
107
107
1:16
Range (%)
90-105
91-102
90-102
Average (%)
96
97
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
96-112
104
Cell culture media (n=5)
95-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.31
6.82
0.94
2.2
7.17
Standard deviation
0.1
0.22
0.55
0.08
0.25
0.54
C V (%)
10.64
9.52
8.06
8.51
11.36
7.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed.
