Human FUT3 Recombinant Protein (RPPB1653)
- SKU:
- RPPB1653
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21217
- Research Area:
- Enzymes
Description
| Product Name: | Human FUT3 Recombinant Protein |
| Product Code: | RPPB1653 |
| Size: | 10µg |
| Species: | Human |
| Target: | FUT3 |
| Synonyms: | Galactoside 3(4)-L-fucosyltransferase, Blood group Lewis alpha-4-fucosyltransferase, Lewis FT, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3, FT3B, LE, CD174, Les. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The FUT3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSRVSRDDA TGSPRAPSGS SRQDTTPTRP TLLILLWTWP FHIPVALSRC SEMVPGTADC HITADRKVYP QADTVIVHHW DIMSNPKSRL PPSPRPQGQR WIWFNLEPPP NCQHLEALDR YFNLTMSYRS DSDIFTPYGW LEPWSGQPAH PPLNLSAKTE LVAWAVSNWK PDSARVRYYQ SLQAHLKVDV YGRSHKPLPK GTMMETLSRY KFYLAFENSL HPDYITEKLW RNALEAWAVP VVLGPSRSNY ERFLPPDAFI HVDDFQSPKD LARYLQELDK DHARYLSYFR WRETLRPRSF SWALDFCKAC WKLQQESRYQ TVRSIAAWFT |
Fucosyltransferase 3 (FUT3) catalyzes alpha-1, 3 and alpha-1, 4 glycosidic linkages which take part in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. FUT3 takes part in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. FUT3 also operates on the corresponding 1, 4-galactosyl derivative, creating1, 3-L-fucosyl links.
FUT3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (35-361 a.a) and having a molecular mass of 40.6kDa.FUT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; EC 2.4.1.65 Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: Golgi membrane; membrane Molecular Function:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity Biological Process: biopolymer glycosylation; cell-cell recognition; oligosaccharide biosynthetic process |
| NCBI Summary: | The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P21217 |
| NCBI GenInfo Identifier: | 121137 |
| NCBI Gene ID: | 2525 |
| NCBI Accession: | P21217.1 |
| UniProt Secondary Accession: | P21217,Q32NE7, Q99448, Q99449, B5U7U9, B5U7V0, |
| UniProt Related Accession: | P21217 |
| Molecular Weight: | 42,117 Da |
| NCBI Full Name: | Galactoside 3(4)-L-fucosyltransferase |
| NCBI Synonym Full Names: | fucosyltransferase 3 (Lewis blood group) |
| NCBI Official Symbol: | FUT3�� |
| NCBI Official Synonym Symbols: | LE; Les; FT3B; CD174; FucT-III�� |
| NCBI Protein Information: | galactoside 3(4)-L-fucosyltransferase |
| UniProt Protein Name: | Galactoside 3(4)-L-fucosyltransferase |
| UniProt Synonym Protein Names: | Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucT-III |
| Protein Family: | Fucosyltransferase |
| UniProt Gene Name: | FUT3�� |
| UniProt Entry Name: | FUT3_HUMAN |
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