Human GAD2 Recombinant Protein (RPPB1665)
- SKU:
- RPPB1665
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q05329
- Research Area:
- Enzymes
Description
| Product Name: | Human GAD2 Recombinant Protein |
| Product Code: | RPPB1665 |
| Size: | 100µg |
| Species: | Human |
| Target: | GAD2 |
| Synonyms: | Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa), Glutamate Decarboxylase 65 KDa Isoform, 65 KDa Glutamic Acid Decarboxylase, EC 4.1.1.15, GAD-65, GAD65, Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kD), Glutamate Decarboxylase-2 (Pancreas), EC 4.1.1, GAD2. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GAD2 protein solution is supplied in 16mM HEPES buffer pH-7.5, 250mM NaCl, 0.016mM Pyridoxal-5�-Phosphate, 0.16% Triton x-100 and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
Glutamate Decarboxylase 2 (GAD2) is one of several forms of glutamic acid decarboxylase, identified as a main autoantigen in�type II diabetes. The GAD2 enzyme is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for the GAD2 enzyme has been characterized in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in�type II diabetes. GAD2 gene may also have a role in the stiff man syndrome. In addition, GAD2 catalyzes the production of GABA.
Recombinant Human GAD2 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66,234 Dalton. GAD2 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 10p11.23 Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cytosol; cell junction Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process |
| NCBI Summary: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| UniProt Code: | Q05329 |
| NCBI GenInfo Identifier: | 1352216 |
| NCBI Gene ID: | 2572 |
| NCBI Accession: | Q05329.1 |
| UniProt Related Accession: | Q05329 |
| Molecular Weight: | |
| NCBI Full Name: | Glutamate decarboxylase 2 |
| NCBI Synonym Full Names: | glutamate decarboxylase 2 |
| NCBI Official Symbol: | GAD2�� |
| NCBI Official Synonym Symbols: | GAD65�� |
| NCBI Protein Information: | glutamate decarboxylase 2 |
| UniProt Protein Name: | Glutamate decarboxylase 2 |
| UniProt Synonym Protein Names: | 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform |
| Protein Family: | Glutamate decarboxylase |
| UniProt Gene Name: | GAD2�� |
| UniProt Entry Name: | DCE2_HUMAN |
