The Human GLUT1 (Glucose Transporter 1) ELISA Kit is specifically designed for the quantitative detection of GLUT1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.GLUT1 is a key protein responsible for facilitating the transport of glucose across cell membranes, playing a crucial role in glucose metabolism and energy production. Abnormalities in GLUT1 expression have been linked to a range of diseases, including diabetes, cancer, and neurological disorders, underscoring its significance as a biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Human GLUT1 ELISA Kit provides researchers with a reliable tool for investigating the role of GLUT1 in health and disease, offering valuable insights into the molecular mechanisms underlying various physiological and pathological processes.
Product Name:
Human GLUT1 (Glucose Transporter 1) ELISA Kit
SKU:
HUES02780
Target:
Human GLUT1 (Glucose Transporter 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLUT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLUT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLUT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GLUT1. You can calculate the concentration of Human GLUT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
93-109
96-111
Average (%)
95
100
103
1:4
Range (%)
91-106
87-101
83-96
Average (%)
97
93
90
1:8
Range (%)
93-106
82-98
87-99
Average (%)
99
89
93
1:16
Range (%)
88-104
85-101
84-94
Average (%)
95
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
0.89
4.44
0.5
0.86
4.6
Standard deviation
0.03
0.04
0.19
0.03
0.05
0.17
C V (%)
6.12
4.49
4.28
6.0
5.81
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GLUT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLUT1 in samples. No significant cross-reactivity or interference between Human GLUT1 and analogues was observed.
