Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit
The Human GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of GM-CSF levels in human serum, plasma, and cell culture supernatants. This kit is a valuable tool for researchers studying the role of GM-CSF in various physiological and pathological processes.GM-CSF is a cytokine that plays a crucial role in the regulation of immune responses, hematopoiesis, and inflammation. Aberrant GM-CSF levels have been implicated in autoimmune diseases, cancer, and other inflammatory conditions, making it a key target for therapeutic interventions.
With its high performance and ease of use, the Human GM-CSF ELISA Kit from AssayGenie provides researchers with a reliable and reproducible method for measuring GM-CSF levels in biological samples. Whether investigating disease mechanisms or evaluating potential therapies, this kit offers researchers a valuable tool for advancing their research.
Product Name:
Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit
SKU:
HUES01368
Target:
Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GM-CSF. You can calculate the concentration of Human GM-CSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
97-115
90-105
Average (%)
101
105
96
1:4
Range (%)
90-101
81-94
88-98
Average (%)
95
86
93
1:8
Range (%)
90-104
84-96
82-96
Average (%)
95
89
88
1:16
Range (%)
94-106
80-94
84-96
Average (%)
99
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.18
75.65
183.61
25.92
75.14
168.57
Standard deviation
1.67
4.06
9.88
1.4
3.32
5.16
C V (%)
6.63
5.37
5.38
5.4
4.42
3.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GM-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GM-CSF in samples. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.
