The Human GNLY (Granulysin) ELISA Kit is specifically designed for the precise measurement of granulysin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it an excellent choice for various research purposes.Granulysin is a key protein involved in immune responses, particularly in the elimination of pathogens and cancer cells. Its role in the immune system makes it a valuable biomarker for studying infectious diseases, autoimmune disorders, and cancer progression. The Human GNLY ELISA Kit provides researchers with a reliable tool for investigating the role of granulysin in various health conditions and potential therapeutic targets.
Product Name:
Human GNLY (Granulysin) ELISA Kit
SKU:
HUES02622
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.14 ng/mL
Detection range:
0.23-15 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
