Human GSTA4 Recombinant Protein (RPPB1759)
- SKU:
- RPPB1759
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O15217
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTA4 Recombinant Protein |
| Product Code: | RPPB1759 |
| Size: | 10µg |
| Species: | Human |
| Target: | GSTA4 |
| Synonyms: | Glutathione S-transferase A4, GST class-alpha member 4, Glutathione S-transferase A4-4, GSTA4, GSTA4-4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The GSTA4 solution (1mg/ml) contains 20mM Tris-HCl buffer, pH8.0, 20% glycerol, 2mM DTT and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQDGNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDDQQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTVKLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP |
| Biological Activity: | Specific activity is > 4,000 pmol/min/ug, and isdefined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4- dinitrobenzene(CDNB) with reduced glutathione per minute at pH 6.5 at 25C. |
Glutathione S-transferase A4 (GSTA4) is a member of the GST superfamily. The GSTA4 enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows an especially high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is extremely effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, which is an important product of peroxidative degradation of arachidonic acid and a frequently used biomarker for oxidative damage in tissue. The GSTA4 enzyme is expressed at a high level in the brain, placenta, and skeletal muscle and much lower in the lung and liver.
GSTA4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (1-222) and having a molecular mass of 28.3kDa.GSTA4 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTA4: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4- hydroxynonenal (4-HNE). Belongs to the GST superfamily. Alpha family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Transferase Chromosomal Location of Human Ortholog: 6p12.1 Cellular Component: cytosol Molecular Function:glutathione transferase activity; protein binding; protein homodimerization activity Biological Process: glutathione metabolic process; xenobiotic metabolic process |
| NCBI Summary: | Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15217 |
| NCBI GenInfo Identifier: | 6016167 |
| NCBI Gene ID: | 2941 |
| NCBI Accession: | O15217.1 |
| UniProt Secondary Accession: | O15217,Q5T7Q8, Q6P4G1, Q9BX18, Q9H414, B2RD15, |
| UniProt Related Accession: | O15217 |
| Molecular Weight: | 14,884 Da |
| NCBI Full Name: | Glutathione S-transferase A4 |
| NCBI Synonym Full Names: | glutathione S-transferase alpha 4 |
| NCBI Official Symbol: | GSTA4�� |
| NCBI Official Synonym Symbols: | GTA4; GSTA4-4�� |
| NCBI Protein Information: | glutathione S-transferase A4 |
| UniProt Protein Name: | Glutathione S-transferase A4 |
| UniProt Synonym Protein Names: | GST class-alpha member 4; Glutathione S-transferase A4-4 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTA4�� |
| UniProt Entry Name: | GSTA4_HUMAN |
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