Human GSTO1 Mutant Recombinant Protein (RPPB1771)
- SKU:
- RPPB1771
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P78417
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTO1 Mutant Recombinant Protein |
| Product Code: | RPPB1771 |
| Size: | 10µg |
| Species: | Human |
| Target: | GSTO1 Mutant |
| Synonyms: | Glutathione S-transferase omega-1, GSTO 1-1, GSTO1, GSTTLP28, P28, DKFZp686H13163. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GSTO1 protein is supplied in 1x PBS and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
GSTO1 belongs to the theta class glutathione S-transferase-like (GSTTL) protein family. GSTO1 is expressed in a broad array of human tissues and exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities as well as catalyzing the reduction of monomethylarsonate which is an intermediate in the pathway of arsenic biotransformation. Furthermore, GSTO1 shields from oxidative stress which is a risk factor for Alzheimer disease, vascular dementia and stroke. GSTO1 is abundant in alveolar macrophages and airway secretions, with the levels reduced in chronic obstructive pulmonary disease patients. In mouse, the GSTO1 protein acts as a small stress response protein, probably involved in cellular redox homeostasis.
Several polymorphisms in the coding regions of the human GSTO1 have been identified. A polymorphism causing an alanine-to-aspartate (A140D) substitution in amino acid 140 produces a variant with lowered enzyme activities in the arsenic biotransformation.GSTO1 Variant Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 241 amino acids fragment (1-241) having a total molecular mass of 36kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The GSTO1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTO1: Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. Belongs to the GST superfamily. Omega family. |
| UniProt Protein Details: | Protein type:EC 1.8.5.1; Transferase; EC 1.20.4.2; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450 Chromosomal Location of Human Ortholog: 10q25.1 Cellular Component: cytoplasm; cytosol Molecular Function:glutathione dehydrogenase (ascorbate) activity; glutathione transferase activity; methylarsonate reductase activity; oxidoreductase activity; protein binding Biological Process: glutathione metabolic process; L-ascorbic acid metabolic process; methylation; positive regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic catabolic process |
| NCBI Summary: | The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P78417 |
| NCBI GenInfo Identifier: | 6016173 |
| NCBI Gene ID: | 9446 |
| NCBI Accession: | P78417.2 |
| UniProt Secondary Accession: | P78417,Q5TA03, Q7Z3T2, D3DRA3, F5H7H0, |
| UniProt Related Accession: | P78417 |
| Molecular Weight: | 24,796 Da |
| NCBI Full Name: | Glutathione S-transferase omega-1 |
| NCBI Synonym Full Names: | glutathione S-transferase omega 1 |
| NCBI Official Symbol: | GSTO1�� |
| NCBI Official Synonym Symbols: | P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21�� |
| NCBI Protein Information: | glutathione S-transferase omega-1 |
| UniProt Protein Name: | Glutathione S-transferase omega-1 |
| UniProt Synonym Protein Names: | Glutathione S-transferase omega 1-1; GSTO 1-1; Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTO1�� |
| UniProt Entry Name: | GSTO1_HUMAN |
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