The Human Gastrin ELISA Kit is specifically designed for the precise measurement of gastrin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Gastrin is a key hormone that regulates the release of gastric acid in the stomach, playing a critical role in digestion and gut function.
Abnormal levels of gastrin have been linked to various gastrointestinal disorders, including gastric ulcers, gastrinomas, and gastric cancer, making it a valuable biomarker for investigating these conditions and developing potential treatments.Overall, the Human Gastrin ELISA Kit provides researchers with a reliable tool for studying gastrin biology and its implications in gastrointestinal health and disease.
Product Name:
Human GT (Gastrin) ELISA Kit
SKU:
HUES02977
Target:
Human GT (Gastrin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GT. During the reaction, GT in the sample or standard competes with a fixed amount of GT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
91-103
94-110
Average (%)
101
98
102
1:4
Range (%)
89-103
94-107
94-107
Average (%)
96
99
99
1:8
Range (%)
88-98
89-102
99-114
Average (%)
93
94
106
1:16
Range (%)
87-99
86-100
94-106
Average (%)
93
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
91-109
99
Cell culture media (n=5)
92-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.1
130.6
493.4
49.5
142.9
454.1
Standard deviation
3
5.9
20.7
3.4
8.6
18.2
C V (%)
6.37
4.52
4.2
6.87
6.02
4.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GT in samples. No significant cross-reactivity or interference between GT and analogues was observed.
