Human GTF2A1 (General Transcription Factor IIA, Polypeptide 1) CLIA Kit
The Human GTF2A1 (General Transcription Factor IIA Polypeptide 1) CLIA Kit is an advanced diagnostic tool designed for the accurate measurement of GTF2A1 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it an invaluable resource for a variety of research purposes.GTF2A1 is a key transcription factor that plays a crucial role in regulating gene expression and transcription initiation. Its dysregulation has been associated with various diseases, including cancer, metabolic disorders, and developmental abnormalities.
By detecting and quantifying GTF2A1 levels, researchers can gain insights into the molecular mechanisms underlying these conditions and explore potential therapeutic interventions.Whether investigating gene regulation, biomarker discovery, or disease mechanisms, the Human GTF2A1 CLIA Kit offers a reliable and efficient solution for studying the role of GTF2A1 in human biology. Trust in this kit to facilitate accurate and meaningful research findings in the field of transcriptional regulation.
Product Name:
Human GTF2A1 (General Transcription Factor IIA, Polypeptide 1) CLIA Kit
SKU:
HUES00288
Target:
Human GTF2A1 (General Transcription Factor IIA, Polypeptide 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GTF2A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GTF2A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GTF2A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GTF2A1. You can calculate the concentration of Human GTF2A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
93-106
88-101
Average (%)
101
100
94
1:4
Range (%)
95-110
92-104
101-117
Average (%)
102
98
108
1:8
Range (%)
99-111
102-118
99-109
Average (%)
104
109
104
1:16
Range (%)
102-116
90-105
89-99
Average (%)
108
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
99-119
108
Cell culture media (n=5)
97-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
276.73
541.51
2290.84
294.81
591.43
2151.34
Standard deviation
26.73
54.58
175.48
36.26
43.06
212.98
C V (%)
9.66
10.08
7.66
12.3
7.28
9.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GTF2A1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GTF2A1 in samples. No significant cross-reactivity or interference between Human GTF2A1 and analogues was observed.
