Human HAAO Recombinant Protein (RPPB1789)
- SKU:
- RPPB1789
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P46952
- Research Area:
- Enzymes
Description
| Product Name: | Human HAAO Recombinant Protein |
| Product Code: | RPPB1789 |
| Size: | 10µg |
| Species: | Human |
| Target: | HAAO |
| Synonyms: | 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-HAO, 3-hydroxyanthranilic acid dioxygenase, HAD, HAAO, HAO. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | HAAO protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMERRLG VRAWVKENRG SFQPPVCNKL MHQEQLKVMF IGGPNTRKDY HIEEGEEVFY QLEGDMVLRV LEQGKHRDVV IRQGEIFLLP ARVPHSPQRF ANTVGLVVER RRLETELDGL RYYVGDTMDV LFEKWFYCKD LGTQLAPIIQ EFFSSEQYRT GKPIPDQLLK EPPFPLSTRS IMEPMSLDAW LDSHHRELQA GTPLSLFGDT YETQVIAYGQ GSSEGLRQNV DVWLWQLEGS SVVTMGGRRL SLAPDDSLLV LAGTSYAWER TQGSVALSVT QDPACKKPLG |
HAAO is a monomeric cytosolic protein which is a member of intramolecular dioxygenases family containing nonheme ferrous iron. The HAAO protein catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is facilitated by its ability to activate glutamate N-methyl-D-aspartate receptors. Amplified cerebral levels of QUIN may contribute to the pathogenesis of neurologic and inflammatory disorders. HAAO is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system.
HAAO Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (1-286 a.a.) and having a molecular mass of 35kDa.HAAO is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HAAO: Catalyzes the oxidative ring opening of 3- hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate. Belongs to the 3-HAO family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.13.11.6 Chromosomal Location of Human Ortholog: 2p21 Cellular Component: mitochondrial membrane; cytosol Molecular Function:electron carrier activity; ferrous iron binding; oxygen binding; 3-hydroxyanthranilate 3,4-dioxygenase activity Biological Process: anthranilate metabolic process; response to cadmium ion; response to zinc ion; quinolinate biosynthetic process; tryptophan catabolic process |
| NCBI Summary: | 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46952 |
| NCBI GenInfo Identifier: | 308153402 |
| NCBI Gene ID: | 23498 |
| NCBI Accession: | P46952.2 |
| UniProt Secondary Accession: | P46952,Q53QZ7, Q8N6N9, A6NE56, B4DIN2, |
| UniProt Related Accession: | P46952 |
| Molecular Weight: | 17,275 Da |
| NCBI Full Name: | 3-hydroxyanthranilate 3,4-dioxygenase |
| NCBI Synonym Full Names: | 3-hydroxyanthranilate 3,4-dioxygenase |
| NCBI Official Symbol: | HAAO�� |
| NCBI Official Synonym Symbols: | HAO; 3-HAO�� |
| NCBI Protein Information: | 3-hydroxyanthranilate 3,4-dioxygenase; HAD; 3-hydroxyanthranilate oxygenase; 3-hydroxyanthranilic acid dioxygenase |
| UniProt Protein Name: | 3-hydroxyanthranilate 3,4-dioxygenase |
| UniProt Synonym Protein Names: | 3-hydroxyanthranilate oxygenase; 3-HAO; 3-hydroxyanthranilic acid dioxygenase; HAD |
| UniProt Gene Name: | HAAO�� |
| UniProt Entry Name: | 3HAO_HUMAN |
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