The Human HGF (Hepatocyte Growth Factor) ELISA Kit is specifically designed for the precise quantification of HGF levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for a variety of research applications.Hepatocyte Growth Factor is a critical protein involved in cell growth, tissue regeneration, and wound healing. It plays a key role in liver regeneration and has potential implications in various diseases, including cancer and liver disorders.
By measuring HGF levels, researchers can gain insights into disease progression and potential therapeutic interventions.Overall, the Human HGF ELISA Kit from Assay Genie provides researchers with a reliable tool for studying the role of HGF in health and disease, offering valuable information for advancing medical research and developing new treatment strategies.
Product Name:
Human HGF (Hepatocyte Growth Factor) ELISA Kit
SKU:
HUES01370
Target:
Human HGF (Hepatocyte Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HGF. You can calculate the concentration of Human HGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
87-98
84-99
Average (%)
96
92
91
1:4
Range (%)
99-114
85-95
97-113
Average (%)
106
90
103
1:8
Range (%)
100-115
82-94
93-109
Average (%)
108
87
99
1:16
Range (%)
100-115
80-94
98-111
Average (%)
106
87
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
402.4
760.6
3186.1
427.7
796.3
3193.8
Standard deviation
24.1
44.1
140.2
28.7
44.6
115.0
C V (%)
5.99
5.8
4.4
6.71
5.6
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HGF in samples. No significant cross-reactivity or interference between Human HGF and analogues was observed.
