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Human HLA-DRA Recombinant Protein (RPPB3689)

SKU:
RPPB3689
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P01903
€273
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Description

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Product Name:Human HLA-DRA Recombinant Protein
Product Code:RPPB3689
Size:20µg
Species:Human
Target:HLA-DRA
Synonyms:Major Histocompatibility Complex, Class II, DR Alpha, HLA-DRA1,MHC Class II AntigenDRA, MLRW, Histocompatibility AntigenHLA-DRAlpha, HLAClass II Histocompatibility Antigen, DR Alpha Chain, MHC CellSurface Glycoprotein, HLA-DRA1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:HLA-DRA protein solution (0.5mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greaterthan 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MIKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGALANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVTTGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE

Major histocompatibility complex, class II, DR alpha (HLA-DRA) belongsto the MHC class II family. HLA-DRA binds peptides derived from antigens whichaccess the endocytic route of antigen presenting cells (APC) and presents themon the cell surface for identification by the CD4 T-cells. The peptide bindingcleft accommodates peptides of 10-30 residues. The peptides presented by MHCclass II molecules are generated mainly by degradation of proteins which accessthe endocytic route, where they are processed by lysosomal proteases and otherhydrolases.

HLA-DRA Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 212 amino acids (26-216 a.a) andhaving a molecular mass of 24.3kDa.HLA-DRA is fused to a 21 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:HLA-DRA: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; lysosome; lysosomal membrane; late endosome membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; MHC class II protein complex

Molecular Function:MHC class II receptor activity; peptide antigen binding

Biological Process: peptide antigen assembly with MHC class II protein complex; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; cognition; T cell receptor signaling pathway; antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; polysaccharide assembly with MHC class II protein complex

Disease: Hepatitis B Virus, Susceptibility To

NCBI Summary:HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Jul 2008]
UniProt Code:P01903
NCBI GenInfo Identifier:122206
NCBI Gene ID:3122
NCBI Accession:P01903.1
UniProt Related Accession:P01903
Molecular Weight:
NCBI Full Name:HLA class II histocompatibility antigen, DR alpha chain
NCBI Synonym Full Names:major histocompatibility complex, class II, DR alpha
NCBI Official Symbol:HLA-DRA��
NCBI Official Synonym Symbols:HLA-DRA1��
NCBI Protein Information:HLA class II histocompatibility antigen, DR alpha chain
UniProt Protein Name:HLA class II histocompatibility antigen, DR alpha chain
UniProt Synonym Protein Names:MHC class II antigen DRA
Protein Family:Draxin
UniProt Gene Name:HLA-DRA��
UniProt Entry Name:DRA_HUMAN

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